Only adult hemoglobin is produced in fetal nonerythroid x MEL cell hybrids

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Only adult hemoglobin is produced in fetal nonerythroid x MEL cell hybrids.

In order to test whether the ontogenetic origin of human parental cells influences the expression of globin genes, we did fusions of mouse erythroleukemia (MEL) cells with fetal or adult nonerythroid cells and we assessed globin expression in hybrids. For selection of hybrid clones we used a monoclonal antibody that recognizes a human chromosome 11-linked cell surface marker. Globin expression ...

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Aggregation of adult and fetal hemoglobin by ingested nitrate anions

The ingested nitrates sourced from tap water, food, chemicals and pharmaceuticals areconverted to nitrites in the body surfaces by bacteria and then, the nitrite ions can lead thestructural changing in hemoglobin. In the present work, aggregation of the purified hemoglobinin adult (HbA) and in fetus or newborn (HbF) in the presence of nitrite ions were studied.Hemoglobin aggregation was perform...

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Switch from fetal to adult hemoglobin is associated with a change in progenitor cell population.

To examine the switch from fetal to adult hemoglobin at the cellular level, erythroid progenitor cells from newborn infants and adults were cultured in methyl cellulose with erythropoietin. Individual erythroid colonies were labeled with [3H]leucine at various times, and globin synthesis patterns examined by gel electrophoresis and fluorography. The percent gamma- or beta-globin synthesis was d...

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Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors: differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell.

Single-cell microscopic immunodiffusion assays were used to determine the cellular mechanisms that regulate fetal hemoglobin (HbF) levels in cultures of primitive and late erythroid precursors obtained from human adult bone marrow. Two variables--the percentage of cells containing HbF (F cells) and the picograms (pg) of HbF/F cell--were assayed in cells derived from erythroid colony-forming uni...

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Fetal calf serum contains activities that induce fetal hemoglobin in adult erythroid cell cultures.

Cultures of peripheral blood or bone marrow erythroid progenitors display stimulated production of fetal hemoglobin. We investigated whether this stimulation is due to factors contained in the sera of the culture medium. Comparisons of gamma/gamma + beta biosynthetic ratios in erythroid colonies grown in fetal calf serum (FCS) or in charcoal treated FCS (C-FCS) showed that FCS-grown cells had s...

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ژورنال

عنوان ژورنال: Blood

سال: 1986

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v68.6.1384.bloodjournal6861384